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One unit of SUMO Protease cleaves ≥85% of 2 μg control substrate in 1 h at 30°C
with C-terminal 10*His VPGEEKQPVPTPDLVEAEVGSTALLKCGPSRASGNFSQVDWFLIHKERQILIFRVHQGKGQREPGEYEHRLSLQDSVATLALSHVTPHDERMFLCKSKRPRLQDHYVELQVFKAPEEPTIQANVVGIHVDRQELREVATCVGRNGYPIPQVLWYKNSLPLQEEENRVHIQSSQIVESSGLYTLKSVLSARLVKEDKDAQFYCELSYRLPSGNHMKESKEVTVPVFYPAEKVWVEVEPVGLLKEGDHVTIRCLTDGNPQPHFTINKKDPSTGEMEEESTDENGLLSLEPAEKHHSGLYQCQSLDLETTITLSSDPLELLVNYVSDVQVNPTAPEVQEGESLTLTCEAESNQDLEFEWLRDKTGQLLGKGPVLQLNNVRREAGGRYLCMASVPRVPGLNRTQLVSVGIFGSPWMALKERKVWVQENAVLNLSCEASGHPQPTISWNVNGSATEWNPDPQTVVSTLNVLVTPELLETGAECTASNSLGSNTTTIVLKLVTLTTLIPDSSQTTGLSTLTVSPHTRANSTSTEKKLPQPESKGVVGGGSGGGSHHHHHHHHHH Expression System HEK293 Molecular Weight 80-100kDa Purity >95% by SDS-PAGE Endotoxin <0
where the dimers are connected by disulfide bonds
The specific anti-human COL7 antibody was coated in a 96-well microplate
protein T4 Usage Sample collection preparation and preservation
AKT2 Recombinant Rabbit mAb (S-3256) Other kits One unit of SUMO ProteaseProduct Specification Host Rabbit Antigen AKT2 Synonyms RAC beta serine threonine protein kinase; Protein kinase Akt 2; Protein kinase B beta (PKB beta); RAC protein kinase beta (RAC PK beta) Location Cytoplasm, Nucleus Accession P31751 Clone Number S 3256 Antibody Type Recombinant mAb Isotype IgG Application WB Reactivity Hu, Ms, Rt, Mk Positive Sample MEF, Jurkat, JAR, HeLa, NIH 3T3, C6, COS 7 Purification Protein A Concentration 0. 5 mg ml