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The recombinant HA tag fusion proteins can be purified directly from pre-treated cell lysate using Rabbit Anti-HA Agarose Beads
and reverse transcription of cDNA above 6 kb is recommended for 60 min
This system
Il-1α And TNF are both acute phase cytokines with Pro febrile and inflammatory effects
Mouse INOS ELISA Kit Size:96T let it sit for 20Product Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
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