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Incubate in a thermal circulator with the lid set at 100°C and incubate at 95°C for 3 minutes
These two proteins bind directly to each other via the C-terminus of KCa1
Host Mouse Antigen CD8a Synonyms T-lymphocyte differentiation antigen T8/Leu-2
with C-terminal 10*His VPGEEKQPVPTPDLVEAEVGSTALLKCGPSRASGNFSQVDWFLIHKERQILIFRVHQGKGQREPGEYEHRLSLQDSVATLALSHVTPHDERMFLCKSKRPRLQDHYVELQVFKAPEEPTIQANVVGIHVDRQELREVATCVGRNGYPIPQVLWYKNSLPLQEEENRVHIQSSQIVESSGLYTLKSVLSARLVKEDKDAQFYCELSYRLPSGNHMKESKEVTVPVFYPAEKVWVEVEPVGLLKEGDHVTIRCLTDGNPQPHFTINKKDPSTGEMEEESTDENGLLSLEPAEKHHSGLYQCQSLDLETTITLSSDPLELLVNYVSDVQVNPTAPEVQEGESLTLTCEAESNQDLEFEWLRDKTGQLLGKGPVLQLNNVRREAGGRYLCMASVPRVPGLNRTQLVSVGIFGSPWMALKERKVWVQENAVLNLSCEASGHPQPTISWNVNGSATEWNPDPQTVVSTLNVLVTPELLETGAECTASNSLGSNTTTIVLKLVTLTTLIPDSSQTTGLSTLTVSPHTRANSTSTEKKLPQPESKGVVGGGSGGGSHHHHHHHHHH Expression System HEK293 Molecular Weight 80-100kDa Purity >95% by SDS-PAGE Endotoxin <0
including serines 235 and 236
N-Ras (mutated Q61R) Recombinant Rabbit mAb (S-R155) Size:100μl Incubate in a thermal circulatorProduct Specification Host Rabbit Synonyms GTPase NRas, Transforming protein N Ras Location Cell membrane, Golgi apparatus membrane Accession P01111 Clone Number S R155 Antibody Type Recombinant mAb Isotype IgG Application WB, IHC P Reactivity Hu Purification Protein A Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05%BSA, 0. 03% Proclin 300 Stability & Storage 12 months from date of