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(3) washing the cells twice with PBS and discarding the PBS
Gelatinase B
determine the hole in the 450 - nm wavelength
GBP28 Accession Q15848 Amino Acid Sequence ETTTQGPGVLLPLPKGACTGWMAGIPGHPGHNGAPGRDGRDGTPGEKGEKGDPGLIGPKGDIGETGVPGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFAYHITVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGERNGLYADNDNDSTFTGFLLYHDTNGGGSHHHHHH Expression System HEK293 Molecular Weight 36 kDa(Reducing) Purity ＞95%
12-200pg/mL
GFP His Tag protein, Aequorea victoria Size:50μg (3) washing the cells twiceProduct Specification Species Aequorea victoria Antigen GFP Synonyms GFP Protein Accession AAB65663 Amino Acid Sequence Ser2 Lys238, with N terminal 8*His HHHHHHHHSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK Expression System E. coli Molecular Weight 27. 8kDa
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