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13(1):299-316
The color development solution of the substrate should be colorless or very light in color
The capping reaction can generally be completed within 60 min
Ferritin is directly implicated in less common but potentially devastating human diseases including sideroblastic anemias
Proteins in this family are also considered to be members of the damage-associated molecular pattern (DAM) family
StartScript® One Step RT-PCR Kit Other Cytokines 13(1):299-316Product Specification Stability & Storage Store at 25 ~ 15 for 2 years Reference Components Component UA070126 50 Rxns UA070126 250 Rxns One Step Enzyme Mix (25X) 100 l 5 100 l One Step Reaction Mix (2X) 2 625 l 10 625 l RNase Free dH2O 2 1 ml 10 1 ml Protocol 1. Prepare the following mixture in an RNase free centrifuge tube: Component Volume One Step Enzyme Mix (25X) 2 l One Step Reaction Mix (2X) 25 l Gene specific Forward Primer (10 M) 2 l Gene