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the supernatant was discarded by centrifugation at 300 g for 5 min
The microplate was coated with purified rat fT4 to prepare a solid phase carrier
Frizzled-10 is also up-regulated in several cancers and transformed cell lines
The prepared luminescent detection solution was transferred to the protein membrane with a pipette so that it was uniformly covered and incubated for 2 min at room temperature
5mg/ml according to the size in ultrapure water after rapid centrifugation
Mouse CD19 ELISA Kit CD Molecular Antigen the supernatant was discarded byProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and