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and HRP conjugate are sequentially added to microwells pre-coated with an integrin beta 1 (ITGb1) capture antibody
T cell chemotactic factor
PDGF-AA is expressed by monocytes
regulating and maintaining the conformation and function of multiple proteins within cells
the assay is developed using the substrate TMB
Human GPLD1 ELISA Kit Porcine ELISA Kit and HRP conjugate are sequentiallyProduct Specification Usage Self prepared test equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision sampler and gun tip: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 incubator 4. Distilled water or deionized water Sample handling and requirements: Tissue homogenate: Wash the tissue with pre cooled PBS (0. 01 M, pH = 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement